PDIA4 Knockout cell line (Hep G2)

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PDIA4 Knockout cell line (Hep G2) Order

PDIA4 Knockout cell line (Hep G2)
Catalog#
YKO-HT4201
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PDIA4 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PDIA4 Knockout cell line (Hep G2)
Species
Human
Gene Name
PDIA4
Gene ID
9601
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript PDIA4-204)
Validation: PCR+Sanger Sequencing
PDIA4 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PDIA4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PDIA4 gene knockout PDIA4 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PDIA4 Knockout cell line (Hep G2)
PDIA4 Knockout cell line (Hep G2)
Catalog#
YKO-HT4201
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Gene Knockout kit

EZ-cryo™ cell freezing medium

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  • PDIA4 Knockout cell line (A549)

    PDIA4 Knockout cell line (A549)
      inquiry   More details

    Organism: Human
    Gene Name: PDIA4
    Gene ID: 9601
    Size: 1*10^6
    Catalog#: YKO-H682
    YKO-H682(A549)
  • PDIA4 Knockout cell line (HeLa)

    PDIA4 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: PDIA4
    Gene ID: 9601
    Size: 1*10^6
    Catalog#: YKO-HS4247
    YKO-HS4247(HeLa)
  • PDIA4 Knockout cell line (HCT 116)

    PDIA4 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: PDIA4
    Gene ID: 9601
    Size: 1*10^6
    Catalog#: YKO-HS3124
    YKO-HS3124(HCT 116)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • PDIA4 Knockout cell line (A549)

    PDIA4 Knockout cell line (A549)

    Organism: Human
    Gene Name: PDIA4
    Gene ID: 9601
    Size: 1*10^6
    Catalog#: YKO-H682
      inquiry   More details
  • PDIA4 Knockout cell line (HeLa)

    PDIA4 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: PDIA4
    Gene ID: 9601
    Size: 1*10^6
    Catalog#: YKO-HS4247
      inquiry   More details
  • PDIA4 Knockout cell line (HCT 116)

    PDIA4 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: PDIA4
    Gene ID: 9601
    Size: 1*10^6
    Catalog#: YKO-HS3124
      inquiry   More details

PDIA4 Knockout cell line (Hep G2) Order

PDIA4 Knockout cell line (Hep G2)
Catalog#
YKO-HT4201
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PDIA4 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PDIA4 Knockout cell line (Hep G2)
Species
Human
Gene Name
PDIA4
Gene ID
9601
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript PDIA4-204)
Validation: PCR+Sanger Sequencing
PDIA4 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PDIA4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PDIA4 gene knockout PDIA4 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PDIA4 Knockout cell line (Hep G2)
PDIA4 Knockout cell line (Hep G2)
Catalog#
YKO-HT4201
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • PDIA4 Knockout cell line (A549)

    PDIA4 Knockout cell line (A549)
      inquiry   More details

    Organism: Human
    Gene Name: PDIA4
    Gene ID: 9601
    Size: 1*10^6
    Catalog#: YKO-H682
    YKO-H682(A549)
  • PDIA4 Knockout cell line (HeLa)

    PDIA4 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: PDIA4
    Gene ID: 9601
    Size: 1*10^6
    Catalog#: YKO-HS4247
    YKO-HS4247(HeLa)
  • PDIA4 Knockout cell line (HCT 116)

    PDIA4 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: PDIA4
    Gene ID: 9601
    Size: 1*10^6
    Catalog#: YKO-HS3124
    YKO-HS3124(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • PDIA4 Knockout cell line (A549)

    PDIA4 Knockout cell line (A549)

    Organism: Human
    Gene Name: PDIA4
    Gene ID: 9601
    Size: 1*10^6
    Catalog#: YKO-H682
      inquiry   More details
  • PDIA4 Knockout cell line (HeLa)

    PDIA4 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: PDIA4
    Gene ID: 9601
    Size: 1*10^6
    Catalog#: YKO-HS4247
      inquiry   More details
  • PDIA4 Knockout cell line (HCT 116)

    PDIA4 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: PDIA4
    Gene ID: 9601
    Size: 1*10^6
    Catalog#: YKO-HS3124
      inquiry   More details

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